Invention Grant
US07718786B2 Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
有权
在体外获得重组核苷酸序列的方法，由此获得的序列和序列的文库
- Patent Title: Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
- Patent Title (中): 在体外获得重组核苷酸序列的方法，由此获得的序列和序列的文库
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Application No.: US11143455Application Date: 2005-06-03
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Publication No.: US07718786B2Publication Date: 2010-05-18
- Inventor: Daniel Dupret , Jean-Michel Masson , Fabrice Lefevre
- Applicant: Daniel Dupret , Jean-Michel Masson , Fabrice Lefevre
- Applicant Address: FR Nimes
- Assignee: Proteus SA
- Current Assignee: Proteus SA
- Current Assignee Address: FR Nimes
- Agency: Hunton & Williams LLP
- Main IPC: C07H21/02
- IPC: C07H21/02 ; C12Q1/68
Abstract:
Ligation-mediated method of recombining polynucleotides in vitro. Polynucleotides from a library are fragmented and the fragments are hybridized to an assembly template. The hybridized fragments are iteratively re-hybridized and ligated until the ends of the hybridized fragments are adjacent to the ends of other hybridized fragments on the assembly template. A final ligation produces recombined polynucleotides.
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