An in-line node locking mechanism for supporting and locking a node within a rack system such forces applied on a node by the node locking mechanism generate consistent output forces. The in-line node locking mechanism utilizes dual swivel nuts and an in-line loading arrangement that evenly distributes forces along the length of a rail system to create evenly distributed forces and avoid the creation of stress points. An actuating screw causes the node locking mechanism to adjust between unlocked and locked positions. In the unlocked position, the mechanism causes the node to be lowered and move freely along the in-line rail system. In the locked position the mechanism causes the node to securely abut the rack system thereby locking the node within the rack system.