It is possible to change a focal position or spot diameter of an optical stimulation laser beam without causing any misalignment of the optical axis, thus precisely applying optical stimulation to a desired position or region on a specimen. The invention provides a laser microscope comprising an observation light path for guiding an observation laser beam; an optical stimulation light path for guiding an optical stimulation laser beam; and a light-path combining unit for combining these light paths; and the laser microscope also comprises, in at least the optical stimulation light path, a focal-position adjusting unit for adjusting a focal position of the laser beam; an optical-axis misalignment detector for detecting an amount of misalignment of an optical axis between the focal-position adjusting unit and the light-path combining unit; and an alignment unit for adjusting an optical axis position on the basis of the amount of misalignment of the optical axis detected by the optical-axis misalignment detector.