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US07749751B2 Optimized T-DNA transfer and vectors therefor 有权
优化的T-DNA转移和载体

Optimized T-DNA transfer and vectors therefor
Abstract:
The present invention relates to T-DNA vectors and methods for obtaining transgenic eukaryotes using said vectors. The transgenic eukaryotes are characterized in that they contain the T-DNA but not the illegitimately transferred vector backbone sequence. This is achieved by modifying the T-DNA borders such that they are more efficiently nicked or such that they allow elimination of illegitimately transferred vector backbone sequences by means of recombination.
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