An adaptive inventory management system and method eliminates or minimizes frequency interference issues within a facility that employs a plurality of RFID readers for purposes of tracking and managing inventory. A middleware server activates and deactivates adjacent RFID readers within the facility based on the location of an item having an RFID tag. The middleware server also queries an inventory database based on the information collected from the RFID tag of the item and generates a command signal based on information retrieved from the database to control the travel route of the vehicle transporting items of inventory within the facility.