Method of detecting a misfire in an internal combustion engine provided with at least two cylinders and a crankshaft, includes: a. producing a measurement signal linked to the crankshaft travel time by supplying a succession of instantaneous values each corresponding to the difference between two successive segment times, each of these segment times corresponding to the time interval separating two characteristic instants of the movement of the pistons in two successive cylinders in the firing order, b. comparing the value of the measurement signal with a threshold value, and c. generating a signal representative of a misfire if the value of the measurement signal crosses the threshold value, the measurement signal having, for each cylinder, an average value that is offset relative to a reference average value, a step of reducing, for each cylinder, this offset, is performed by a high-pass filtering operation on the measurement signal.