A portable power supply module with winding mechanism is disclosed, which includes a shell body, a battery room formed at the shell body for accommodating at least one battery, a connection line, a pulling compartment in the shell body for accommodation of the connection line and a winding mechanism located in the pulling compartment. At least one set of conductive contact plates are provided and are connected to a circuit board to a supply power from the battery. A first end of the connection line is connected to the circuit board and a second end of the connection line is connected to an output connector. When the connection line is pulled and the winding mechanism slides forward to extend the connection line. When the portable power supply module is not in use, the winding mechanism pulls and retracts the connection body in the shell body.