Invention Grant
- Patent Title: Method of assaying substance capable of changing mitochondrial membrane potential
- Patent Title (中): 测定能够改变线粒体膜电位的物质的方法
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Application No.: US11597275Application Date: 2005-05-18
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Publication No.: US07781184B2Publication Date: 2010-08-24
- Inventor: Asami Umeda , Sumiko Nagaki
- Applicant: Asami Umeda , Sumiko Nagaki
- Applicant Address: JP Osaka-shi
- Assignee: Mitsubishi Tanabe Pharma Corporation
- Current Assignee: Mitsubishi Tanabe Pharma Corporation
- Current Assignee Address: JP Osaka-shi
- Agency: Oblon, Spivak, McClelland, Maier & Neustadt, L.L.P.
- Priority: JP2004-147418 20040518
- International Application: PCT/JP2005/009066 WO 20050518
- International Announcement: WO2005/111228 WO 20051124
- Main IPC: C12Q1/00
- IPC: C12Q1/00 ; C12Q1/02
Abstract:
The present invention aims to provide a convenient and high precision evaluation method of a pharmaceutical agent that changes the mitochondrial membrane potential, using a mitochondrial membrane potential of a cultured cell as an index.The present invention provides a method for evaluating the ability of a test substance to change the mitochondrial membrane potential by using, as an index, the change in the mitochondrial membrane potential, which is produced by the addition of an ion permeability regulator on a cellular membrane to a cell culture medium, wherein the change is measured as variation in the fluorescent intensity of a fluorescent dye.
Public/Granted literature
- US20070202486A1 Method Of Assaying Substance Capable Of Changing Mitochondrial Membrane Potential Public/Granted day:2007-08-30
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