A procedure for the preparation of radioisotopes consisting of a first step of electrodepositing a metallic isotope target to be irradiated on a target-holder element, a second step of irradiating the target, a third step of dissolving the target and a fourth set of purifying the radioisotope from the initial metallic isotope and from other possible radioactive and metallic impurities; the electrodeposition step in turn consisting of a dissolution operation in which the isotope to be irradiated is dissolved in a solution of HNO3 with concentration from 0.5 to 2.5 M, a pH buffering operation, and a recirculation operation, in which the solution obtained above is circulated at a rate from 0.5 to 3 within an electrolytic cell during the current output within the cell itself; the electrodeposition of the target to be irradiated occurs within the electrolytic cell during the recirculation operation.