Invention Grant
- Patent Title: Method for amplification of RNA sequences
- Patent Title (中): 扩增RNA序列的方法
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Application No.: US10578552Application Date: 2004-10-27
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Publication No.: US07794986B2Publication Date: 2010-09-14
- Inventor: Birgit Alberta Louisa Maria Deiman , Arnoldina Margaretha Wilhelmina Strijp
- Applicant: Birgit Alberta Louisa Maria Deiman , Arnoldina Margaretha Wilhelmina Strijp
- Applicant Address: NL Boxtel
- Assignee: Biomerieux B.V.
- Current Assignee: Biomerieux B.V.
- Current Assignee Address: NL Boxtel
- Agency: Myers Bigel Sibley & Sajovec, P.A.
- Priority: EP03078568 20031114
- International Application: PCT/EP2004/012190 WO 20041027
- International Announcement: WO2005/047535 WO 20050526
- Main IPC: C12P19/34
- IPC: C12P19/34 ; C07H21/04

Abstract:
The invention relates to a method for amplification of a target RNA sequence, wherein the first primer comprises a hybridizing sequence of 7 to 14 nucleotides, which is capable of binding to a first segment of the target RNA sequence, a transcription enhancing sequence, and an anchor which is capable of binding to a second segment of the target RNA sequence, and/or wherein the second primer comprises a hybridizing sequence of 7 to 14 nucleotides, an amplification enhancing sequence and an anchor which is capable of binding to a second segment of the first single stranded cDNA. The invention further relates to primers for the amplification of target RNA sequences and to a kit comprising one or more of the primers.
Public/Granted literature
- US20080176294A1 Method For Amplification Of Rna Sequences Public/Granted day:2008-07-24
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