Invention Grant
US07795408B2 Method for cloning and expression of Acc65I restriction endonuclease and Acc65I methylase in E. Coli
有权
在大肠杆菌中克隆并表达Acc65I限制性内切核酸酶和Acc65I甲基化酶的方法
- Patent Title: Method for cloning and expression of Acc65I restriction endonuclease and Acc65I methylase in E. Coli
- Patent Title (中): 在大肠杆菌中克隆并表达Acc65I限制性内切核酸酶和Acc65I甲基化酶的方法
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Application No.: US11818298Application Date: 2007-06-14
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Publication No.: US07795408B2Publication Date: 2010-09-14
- Inventor: Keith Lunnen , John Greci , Geoffrey Wilson
- Applicant: Keith Lunnen , John Greci , Geoffrey Wilson
- Applicant Address: US MA Ipswich
- Assignee: New England Biolabs, Inc.
- Current Assignee: New England Biolabs, Inc.
- Current Assignee Address: US MA Ipswich
- Agent Harrier M. Strimpel
- Main IPC: C07H21/02
- IPC: C07H21/02 ; C07H21/04 ; C12P21/06 ; C12N1/20 ; C12N15/74
Abstract:
An isolated DNA is provided which encodes a protein that is capable of binding to 5′GGTACC-3′, the isolated DNA being capable of hybridizing to SEQ ID NO:3 under stringent hybridization conditions. The isolated DNA may be alternatively characterized as coding for a protein having an amino acid sequence comprising SEQ ID NO:5 or by an amino acid sequence with an expectation value of less than E=e−02 in a BLAST search using SEQ ID NO:5. Vectors containing the isolated DNA and host cells expressing the vectors as well as a method for making recombinant Acc65I having the above properties are also provided.
Public/Granted literature
- US20080009036A1 Method for cloning and expression of Acc65I restriction endonuclease and Acc65I methylase in E. coli Public/Granted day:2008-01-10
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