A laminate of a thermoplastic resin and rubber comprising a thermoplastic resin layer (A) made of a matrix of a polyamide resin matrix containing an elastomer dispersed therein and an unvulcanized diene-based rubber layer (B) containing a diene-based rubber and a low molecular weight diene-based rubber having an acid anhydride group and having a weight average molecular weight of 500 to 50,000 laminated partially or totally in at least one layer each to form a laminate integrated at the time of vulcanization and a pneumatic tire using the same.