The present system for analyzing eye responses accurately and automatically tracks the size, location, and movement of a subject's pupil(s) in response to a predetermined test protocol. These eye responses include both ocularmotor and pupillary responses to illumination and target tracking for either eye or both eyes simultaneously. The series of digital image frames of a subject's eyes, taken during the execution of a predetermined stimulus protocol, are automatically processed to determine pupil location, size, and responsiveness to changes in illumination.