A method and system for detecting spectral Doppler blood flow velocity are disclosed. The method comprises the steps of: obtaining Doppler signals of the flow by demodulating, filtering, and analog-to-digital converting RF ultrasonic echoes; analyzing the spectrum of the Doppler signals to obtain each of the power spectral lines of the Doppler signal varying with time; determining a threshold; and determining the frequency shift parameters or the blood flow velocity corresponding to the current power spectral line based on the threshold and the current power spectral line.