A differential-signal output circuit for a timing controller of a display device includes a conversion circuit, a pre-charging circuit and a timing generator. The conversion circuit is used for receiving a differential signal and outputting a current to a load circuit according to polarity of the differential signal. The pre-charging circuit is coupled to a first output end and a second output end of the conversion circuit or is coupled to a first power driving end and a power second driving end of the conversion circuit. The pre-charging circuit is used for pre-charging the load according to a control signal. The timing generator is used for generating the differential signal and a control signal according to display data.