A method of forming a contact hole includes forming a plurality of lower patterns on a substrate. An insulation layer is formed on the lower patterns. A self-assemble induction layer is formed on the insulation layer. A recess is formed in the self-assemble induction layer in alignment with the lower patterns. A block copolymer layer is formed in the recess to form a polymer domain at a distance from a sidewall of the recess and a polymer matrix surrounding the polymer domain. The polymer domain is removed. The self-assemble induction layer is etched using the polymer matrix as a mask to form an opening through the self-assemble induction layer to expose the insulation layer. The insulation layer exposed by the opening is etched using the self-assemble induction layer as a mask so as to form a contact hole.