Invention Grant
- Patent Title: Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof
- Patent Title (中): 衍生自作为染色体复制酶的嗜热生物体的酶及其制备和用途
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Application No.: US12478741Application Date: 2009-06-04
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Publication No.: US07803596B2Publication Date: 2010-09-28
- Inventor: Olga Yurieva , John Kuriyan , Michael E. O'Donnell , David Jeruzalmi
- Applicant: Olga Yurieva , John Kuriyan , Michael E. O'Donnell , David Jeruzalmi
- Applicant Address: US NY New York
- Assignee: The Rockefeller University
- Current Assignee: The Rockefeller University
- Current Assignee Address: US NY New York
- Agency: Nixon Peabody LLP
- Main IPC: C12N9/12
- IPC: C12N9/12 ; C12N1/20
Abstract:
A DNA Polymerase has been identified in a thermophile that functions as a chromosomal replicase. The specific enzyme is a holoenzyme III that has been identified in Thermus thermophilus, and corresponds to Polymerase III in E. coli. The genes and the polypeptides corresponding to T.th. γ, τ, ε, α and β subunits that they encode are disclosed, as are probes, vectors, methods of preparation and the methods of use. The enzymes of the present invention and their components are particularly well suited for use in procedures for the preparation of DNA, such as PCR, because of the speed and accuracy that they are able to achieve.
Public/Granted literature
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