According to the invention, there is provided a novel soybean variety designated RJS56001. This invention thus relates to the seeds of soybean variety RJS56001, to the plants of soybean RJS56001 to plant parts of soybean variety RJS56001 and to methods for producing a soybean plant produced by crossing plants of the soybean variety RJS56001 with another soybean plant, using RJS56001 as either the male or the female parent.