A laser angle guide assembly has a stand, a laser guide and a reflector. The laser guide is mounted adjustably on the stand and has a body, a side index line, a front index line and an index protrusion. The side index line is formed on the side faces of the body. The front index line is formed on the front face of the body. The index protrusion is formed on the front surface at the bottom of the body and has an inclined top and a front face. The reflector is attached and inclined relative to the front surface of the laser guide at an angle and has a reflecting side facing to the inclined top of the index protrusion. Accordingly, the laser angle guide assembly can provide a guide effect to help a doctor to puncture a needle assembly at a desired angle precisely.