Invention Grant
US07807347B2 Immuno-PCR method for detecting nasopharyngeal carcinoma markers and kit thereof
有权
用于检测鼻咽癌标志物的免疫PCR方法及其试剂盒
- Patent Title: Immuno-PCR method for detecting nasopharyngeal carcinoma markers and kit thereof
- Patent Title (中): 用于检测鼻咽癌标志物的免疫PCR方法及其试剂盒
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Application No.: US12232763Application Date: 2008-09-24
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Publication No.: US07807347B2Publication Date: 2010-10-05
- Inventor: Hsiang-Yin Lu , Tzu-Wei Wang , Feng-Huei Lin , Pei-Jen Lou
- Applicant: Hsiang-Yin Lu , Tzu-Wei Wang , Feng-Huei Lin , Pei-Jen Lou
- Applicant Address: TW Taipei
- Assignee: National Taiwan University
- Current Assignee: National Taiwan University
- Current Assignee Address: TW Taipei
- Agency: Bacon & Thomas PLLC
- Priority: TW97102138A 20080121
- Main IPC: C12Q1/70
- IPC: C12Q1/70
Abstract:
The present invention is related to an immuno-PCR method for detecting nasopharyngeal carcinoma (NPC) and kit thereof, especially related to an immuno-PCR method for detecting markers of early stage NPC and kit thereof. The present invention includes providing a substrate whereon protein markers immobilized; applying a patient's specimen to the substrate; adding a solution which has biotinylated anti-human IgA secondary antibody and incubating the solution; adding a solution with a linker and biotinylated target DNA; proceeding a polymerase chain reaction; and finally, detecting the target DNA fragments via electrophoresis.
Public/Granted literature
- US20090186336A1 Immuno-PCR method for detecting nasopharyngeal carcinoma markers and kit thereof Public/Granted day:2009-07-23
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