Invention Grant
US07807404B2 Mutated truncated mt-pfkA gene for the synthesis of active shorter fragment of 6-phosphofructo-1-kinase
有权
用于合成6-磷酸果糖-1-激酶活性较短片段的突变的截短的mt-pfkA基因
- Patent Title: Mutated truncated mt-pfkA gene for the synthesis of active shorter fragment of 6-phosphofructo-1-kinase
- Patent Title (中): 用于合成6-磷酸果糖-1-激酶活性较短片段的突变的截短的mt-pfkA基因
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Application No.: US12298269Application Date: 2007-02-27
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Publication No.: US07807404B2Publication Date: 2010-10-05
- Inventor: Matic Legisa , Mojca Bencina , Gregor Tevz , Maja Capuder , Tina Mlakar , Darija Oven
- Applicant: Matic Legisa , Mojca Bencina , Gregor Tevz , Maja Capuder , Tina Mlakar , Darija Oven
- Applicant Address: SI Ljubljana
- Assignee: Kemijski Institut
- Current Assignee: Kemijski Institut
- Current Assignee Address: SI Ljubljana
- Agency: Smith Moore Leatherwood LLP
- Priority: SI200600107 20060426
- International Application: PCT/SI2007/000007 WO 20070227
- International Announcement: WO2007/123498 WO 20071101
- Main IPC: C12N9/12
- IPC: C12N9/12 ; C12N1/00 ; C07H21/04 ; C12P1/00
Abstract:
The invention deals with mutated truncated mt-pfkA gene encoding shorter fragment of 6-phosphofructo-1-kinase (PFK1), with no need for phosphorylation of the protein molecule for activation, that is not significantly inhibited by citric acid and/or its salts and ATP molecules. Active 49-52 kDa fragment encoded by mt-pfkA gene, retains positive regulatory properties of the native protein and is activated in the presence of specific activators, while citric acid and ATP, important metabolites that function as feed back inhibitors in higher organisms do not reduce its activity. The invention deals with the use of modified shorter fragment in biotechnological processes for fabricating primary and secondary metabolites.
Public/Granted literature
- US20090098605A1 MUTATED TRUNCATED mt-pfkA GENE FOR THE SYNTHESIS OF ACTIVE SHORTER FRAGMENT OF 6-PHOSPHOFRUCTO-1-KINASE Public/Granted day:2009-04-16
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