Invention Grant
- Patent Title: Directed evolution of proteins
- Patent Title (中): 蛋白质的定向进化
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Application No.: US11107335Application Date: 2005-04-15
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Publication No.: US07807408B2Publication Date: 2010-10-05
- Inventor: David R. Liu , Joshua A. Bittker , Jane M. Liu
- Applicant: David R. Liu , Joshua A. Bittker , Jane M. Liu
- Applicant Address: US MA Cambridge
- Assignee: President & Fellows of Harvard College
- Current Assignee: President & Fellows of Harvard College
- Current Assignee Address: US MA Cambridge
- Agency: Nutter McClennen & Fish LLP
- Agent Thomas J. Engellenner
- Main IPC: C12P21/06
- IPC: C12P21/06 ; C12P19/34 ; C12Q1/68 ; C12N1/20 ; C12N15/00
Abstract:
Disclosed is a method of altering a nucleic acid such as RNA or DNA. The method comprises fragmenting a parent nucleic acid strand to generate nucleic acid fragments. At least a subset of the fragments are ligated to generate shuffled nucleic acid strands. A selected strand is identified from the shuffled nucleic acid strands for a criterion. The methods of the invention can also be used to diversify proteins.
Public/Granted literature
- US20050260655A1 Directed evolution of proteins Public/Granted day:2005-11-24
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