Invention Grant
US07807460B2 Expression vector system regulated by σ32 and methods for using it to produce recombinant protein
有权
由sgr 32调控的表达载体系统及其用于产生重组蛋白的方法
- Patent Title: Expression vector system regulated by σ32 and methods for using it to produce recombinant protein
- Patent Title (中): 由sgr 32调控的表达载体系统及其用于产生重组蛋白的方法
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Application No.: US11614626Application Date: 2006-12-21
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Publication No.: US07807460B2Publication Date: 2010-10-05
- Inventor: Weilan Shao , Huawei Wu , Jianjun Pei
- Applicant: Weilan Shao , Huawei Wu , Jianjun Pei
- Agency: Pinsent Masons LLP
- Agent Kening Li
- Main IPC: C12N5/00
- IPC: C12N5/00 ; C12N15/00 ; C12P21/04 ; C07H21/02
Abstract:
This invention discloses an expression vector system comprising a promoter recognized and regulated by a heat shock sigma factor of E. coli, especially σ32. Preferably, the promoter comprises the consensus sequence of E. coli heat shock promoters as shown in SEQ ID NO:1. Also disclosed are methods for producing proteins using the promoter under heat shock conditions. Furthermore, the present invention discloses a method for creating a sudden temperature shift in a cell culture which has been pre-cultured to reach an optimal condition and which temperature shift will allow optimal production of a recombinant protein under the control a heat shock promoter.
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