In described embodiments, a communication system employing, for example, clock and data recovery (CDR) detects and applies correction for DC offset in an input data stream signal, termed as “DC offset calibration”. DC offset calibration applies static calibration for DC offset in input circuits, such as an input amplifier and detection latches, without input data, and applies statistical calibration for DC offset during operation with an input data stream to correct for dynamic shifts in DC level. Such DC offset calibration employs data eye measurements of the input data stream for detection of DC offset, and applies an opposite DC offset to maintain a relatively balanced data eye during live traffic.