Invention Grant
- Patent Title: PCV2 immunogenic compositions and methods of producing such compositions
- Patent Title (中): PCV2免疫原性组合物及其制备方法
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Application No.: US12137960Application Date: 2008-06-12
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Publication No.: US07829101B2Publication Date: 2010-11-09
- Inventor: Marc Eichmeyer , Greg Nitzel , Merrill Schaeffer
- Applicant: Marc Eichmeyer , Greg Nitzel , Merrill Schaeffer
- Applicant Address: US MO Saint Joseph
- Assignee: Boehringer Ingelheim Vetmedica, Inc.
- Current Assignee: Boehringer Ingelheim Vetmedica, Inc.
- Current Assignee Address: US MO Saint Joseph
- Agent Michael P. Morris; Julie A. Scott
- Main IPC: A61K39/12
- IPC: A61K39/12

Abstract:
An improved method for recovering the protein expressed by open reading frame 2 from porcine circovirus type 2 is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame 2 coding sequences into cells contained in growth media, causing the virus to express open reading frame 2, and recovering the expressed protein in the supernate. This recovery should take place beginning approximately 5 days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time-consuming extraction procedures required to separate and recover the recombinant protein from within the cells.
Public/Granted literature
- US20090016992A1 PCV2 IMMUNOGENIC COMPOSITIONS AND METHODS OF PRODUCING SUCH COMPOSITIONS Public/Granted day:2009-01-15
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