Invention Grant
US07829502B2 Systems and methods for nuclease-assisted selection and acquisition of single stranded DNA oligomer/polymer aptamers/ligands
有权
用于核酸酶辅助选择和获得单链DNA寡聚体/聚合物适体/配体的系统和方法
- Patent Title: Systems and methods for nuclease-assisted selection and acquisition of single stranded DNA oligomer/polymer aptamers/ligands
- Patent Title (中): 用于核酸酶辅助选择和获得单链DNA寡聚体/聚合物适体/配体的系统和方法
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Application No.: US12033522Application Date: 2008-02-19
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Publication No.: US07829502B2Publication Date: 2010-11-09
- Inventor: Gregory F. Lopreato
- Applicant: Gregory F. Lopreato
- Main IPC: C40B30/04
- IPC: C40B30/04 ; C40B40/08

Abstract:
A method for identifying aptamers that bind to target molecules may include contacting an oligonucleotide library with target molecule and digesting unbound oligonucleotides with one or more endonucleases, one or more exonucleases, or one or more endonucleases in combination with one or more exonucleases. A method for identifying aptamers may further include optionally subjecting selected aptamers to one or more rounds of selection under conditions of increased stringency. A method for identifying aptamers may include yet further amplifying selected aptamers. The described methods may be performed in a screen for identifying aptamers either alone or in combination with other methods typically employed in the art for selecting aptamers (such as, e.g., SELEX). Also contemplated herein are systems and kits for accomplishing the above.
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