A system-on-a-chip and a power gating circuit thereof are provided. The power gating circuit includes a first transistor, a charge pump circuit, and a hold circuit. A gate terminal of the first transistor is controlled by a first input signal. A first source/drain terminal of the first transistor is coupled to a first voltage. A second source/drain terminal of the first transistor outputs an output voltage. The charge pump circuit is coupled to a bulk terminal of the first transistor for changing a bulk voltage of the first transistor according to a second input signal. The hold circuit is coupled to the bulk terminal of the first transistor for holding the bulk voltage of the first transistor.