An areal active pixel image sensor (AAPS) with programmable row-specific gain is disclosed for converting hyper-spectral light image into video output signal (VOS). The AAPS includes: a) An areal active pixel sensor (APS) array each capable of photoelectrically converting and integrating an incident pixel light into a photoelectric signal through an integration time period TNT with a photoelectric signal gain GPE. b) A video output signal conditioner (VOSC), coupled to the APS array, for multiplexing and amplifying the photoelectric signals into the VOS with an electric signal gain GEE. c) The VOSC further programmably sets at least one of GPE and GEE to be row-specific. Consequently, the AAPS exhibits an overall photoelectric signal gain of GOA=GPE×GEE that is row-specific and it can compensate for image signal distortion caused by non-uniform spectral response of the APS elements during hyper-spectral imaging.