A puncturing guidance tube for a long and fine needle is invented that is comprised of: a cylindrical body forming a long tubal-shape, a head portion forming a funnel shaped rim, an upper portion (31) of the cylindrical body having a large diameter, a lower portion (32) of the cylindrical body having a small diameter, a transition portion disposed between the upper portion and the lower portion, wherein the large diameter is gradually reduced to the small diameter of the cylindrical body, and a needle gateway (33) forming a lateral opening vertically all along the cylindrical body for easily removing the guidance tube from an operating needle, so that the long and fine needle can be punctured without buckling during an initial operation of the puncturing. The overall length of the tube is about two-third of the needle and the opening width of the needle gateway (33) is wide enough to pass out the needle.