A color filter substrate having a substrate, a black matrix layer, a first filter layer and a second filter layer is provided. The black matrix layer is disposed on the substrate, and multiple pixel areas is defined on the substrate by the black matrix layer. Each pixel area includes a first sub-pixel area and a second sub-pixel area. The first and second filter layer are disposed in the pixel area, and the second filter layer covers the first filter layer. A refractive index of the second filter layer is greater than that of the first filter layer, such that light emitting direction of the first sub-pixel area is different from that of the second sub-pixel area. Besides, a fabricating method of the color filter substrate and an LCD panel with the color filter substrate are provided. The LCD panel with 3D image display or dual-view function can be easily manufactured.