A rapid iris acquisition, tracking, and imaging system can be used at longer standoff distances and over larger capture volumes, without the active cooperation of subjects. Light illuminates the subjects' eyes and a high resolution camera captures images of the irises. The images of the irises are processed by a post processing module to improve their quality. In one approach, the point spread function of the image capture subsystem is estimated using glint reflections from the eye, and the estimated point spread function is used in deconvolution to increase the resolution of the iris images. The post processed iris images have sufficient resolution to be used for biometric identification.