A handheld radio frequency identification (RFID) reader includes a polarization insensitive antenna that enables simultaneous operation in two orthogonal polarization modes. The RFID reader includes an RF communication module and an antenna coupled to the RF communication module. The RF communication module is configured to process RF signals associated with operation of the RFID reader, and the antenna is configured to transmit and receive RF energy associated with operation of the RFID reader. The antenna includes a conductive loop element having a major longitudinal path, and a slot formed in the conductive loop element along the major longitudinal path. The conductive loop element is configured to operate as a first antenna polarized in a first orientation, and the slot is configured to operate as a second antenna polarized in a second orientation.