Invention Grant
- Patent Title: Modified polypeptides stabilized in a desired conformation and methods for producing same
- Patent Title (中): 以所需构象稳定的修饰多肽及其制备方法
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Application No.: US12592275Application Date: 2009-11-20
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Publication No.: US07879577B2Publication Date: 2011-02-01
- Inventor: Timothy A. Springer , Motomu Shimaoka , Chafen Lu
- Applicant: Timothy A. Springer , Motomu Shimaoka , Chafen Lu
- Applicant Address: US MA Boston
- Assignee: The Center for Blood Research, Inc.
- Current Assignee: The Center for Blood Research, Inc.
- Current Assignee Address: US MA Boston
- Agency: Rissman Hendricks & Oliverio, LLP
- Main IPC: C12P21/06
- IPC: C12P21/06 ; C12P11/00 ; A61K38/00 ; A61K31/67
Abstract:
The present invention provides a method for stabilizing a protein in a desired conformation by introducing at least one disulfide bond into the polypeptide. Computational design is used to identify positions where cysteine residues can be introduced to form a disulfide bond in only one protein conformation, and therefore lock the protein in a given conformation. Accordingly, antibody and small molecule therapeutics are selected that are specific for the desired conformation. The invention also provides modified integrin I-domain polypeptides that are stabilized in a desired conformation. The invention further provides screening assays and therapeutic methods utilizing the modified integrin I-domains of the invention.
Public/Granted literature
- US20100113742A1 Modified polypeptides stabilized in a desired conformation and methods for producing same Public/Granted day:2010-05-06
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