Invention Grant
- Patent Title: High spatial resolution imaging of a structure of interest in a specimen
- Patent Title (中): 样本中感兴趣结构的高空间分辨率成像
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Application No.: US12128948Application Date: 2008-05-29
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Publication No.: US07880150B2Publication Date: 2011-02-01
- Inventor: Stefan Hell , Christian Eggeling , Alexander Egner , Jonas Fölling , Andreas Schönle , Mariano Bossi
- Applicant: Stefan Hell , Christian Eggeling , Alexander Egner , Jonas Fölling , Andreas Schönle , Mariano Bossi
- Applicant Address: DE München
- Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.
- Current Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.
- Current Assignee Address: DE München
- Agency: Thomas, Kayden, Horstemeyer & Risley, LLP
- Priority: DE102006021317 20060506
- Main IPC: G01N21/64
- IPC: G01N21/64

Abstract:
For the high spatial resolution imaging of a structure of interest in a specimen, a substance is selected from a group of substances which have a fluorescent first state and a nonfluorescent second state; which can be converted fractionally from their first state into their second state by light which excites them into fluorescence, and which return from their second state into their first state; the specimen's structure of interest is imaged onto a sensor array, a spatial resolution limit of the imaging being greater (i.e. worse) than an average spacing between closest neighboring molecules of the substance in the specimen; the specimen is exposed to light in a region which has dimensions larger than the spatial resolution limit, fractions of the substance alternately being excited by the light to emit fluorescent light and converted into their second state, and at least 10% of the molecules of the substance that are respectively in the first state lying at a distance from their closest neighboring molecules in the first state which is greater than the spatial resolution limit; and the fluorescent light, which is spontaneously emitted by the substance from the region, is registered in a plurality of images recorded by the sensor array during continued exposure of the specimen to the light.
Public/Granted literature
- US20090134342A1 High spatial resolution imaging of a structure of interest in a specimen Public/Granted day:2009-05-28
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