A system is provided for characterizing optical fibers carrying signal traffic. The system includes a transmitter, a variable optical attenuator (VOA), a receiver, and a computing device. The transmitter propagates an optical test signal along a channel of a fiber pathway. The VOA adjusts the attenuation of the optical test signal from an initial, greater attenuation to a subsequent, lesser attenuation. At the same time, the computing device monitors at least one other channel of the fiber pathway and identifies effects upon the other channel(s) from the optical test signal. The computing device may communicate with the VOA and with other components of the fiber pathway to direct adjustment of the signal strength. A maximum optical test signal strength may thus be achieved that does not negatively affect signal traffic on the other channels, and the fiber pathway may subsequently be tested using the achieved maximum optical test signal strength.