A dissection apparatus is disclosed. The dissection apparatus includes a supporting mechanism, a holder, a driving element rotating the holder, a cam fixed to the supporting mechanism, a first sliding element, a second sliding element, a dissecting device disposed on the first sliding element, an aspirating device disposed on the second sliding element. When the holder rotates to move the dissecting device from a first idle position to an operating position, the first sliding element is lowered by the guide of the cam enabling the aspirating device to move from the operating position to a second idle position. When the holder rotates to move the aspirating device from the second idle position to the operating position, the guide of the cam lowers the second sliding element, thus, allowing the dissecting device is able to move from the operating position to the first idle position.