Invention Grant
US07888031B2 Detection of polyketide synthetase gene expression in Karenia brevis
有权
Karenia brevis中聚酮化合物合成酶基因表达的检测
- Patent Title: Detection of polyketide synthetase gene expression in Karenia brevis
- Patent Title (中): Karenia brevis中聚酮化合物合成酶基因表达的检测
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Application No.: US12206385Application Date: 2008-09-08
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Publication No.: US07888031B2Publication Date: 2011-02-15
- Inventor: John H. Paul, III
- Applicant: John H. Paul, III
- Applicant Address: US FL Tampa
- Assignee: University of South Florida
- Current Assignee: University of South Florida
- Current Assignee Address: US FL Tampa
- Agency: Saliwanchik, Lloyd & Eisenschenk
- Main IPC: C12Q1/68
- IPC: C12Q1/68
Abstract:
The present invention concerns an assay for the detection of polyketide synthetase (PKS) mRNA from the red tide dinoflagellate Karenia brevis. Another aspect of the invention concerns NASBA primers and a molecular beacon that enable detection of mRNA of one of the PKS genes. In another aspect, the present invention includes an internal control RNA (IC-RNA) that enables quantitative detection of the target as well as correction for inhibition of amplification. The assay of the invention provides a rapid and specific detection of the putative toxin-producing genes of the Florida Red Tide Organism. The assay of the invention is useful for determining the virulence of red tide blooms.
Public/Granted literature
- US20090075286A1 Detection of Polyketide Synthetase Gene Expression in Karenia Brevis Public/Granted day:2009-03-19
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