Invention Grant
US07888064B2 Gram positive bacterial cells comprising a disrupted flagellin gene, flagellin-based fusion proteins and use in removal of metal ions from a liquid
有权
包含破碎的鞭毛蛋白基因,基于鞭毛蛋白的融合蛋白和用于从液体中去除金属离子的革兰氏阳性细菌细胞
- Patent Title: Gram positive bacterial cells comprising a disrupted flagellin gene, flagellin-based fusion proteins and use in removal of metal ions from a liquid
- Patent Title (中): 包含破碎的鞭毛蛋白基因,基于鞭毛蛋白的融合蛋白和用于从液体中去除金属离子的革兰氏阳性细菌细胞
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Application No.: US11792242Application Date: 2005-12-02
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Publication No.: US07888064B2Publication Date: 2011-02-15
- Inventor: Eldie Berger , Maureen Elizabeth Louw , Michael Craig Crampton
- Applicant: Eldie Berger , Maureen Elizabeth Louw , Michael Craig Crampton
- Applicant Address: ZA Scientia, Pretoria
- Assignee: CSIR
- Current Assignee: CSIR
- Current Assignee Address: ZA Scientia, Pretoria
- Agency: Fulwider Patton LLP
- Priority: ZA2004/9786 20041202
- International Application: PCT/IB2005/054022 WO 20051202
- International Announcement: WO2006/072845 WO 20060713
- Main IPC: C12P21/06
- IPC: C12P21/06 ; C07K14/001

Abstract:
The invention provides flagellin-based fusion proteins (FBFP) are useful for a variety of purposes, in bioremediation to remove metal ions from a liquid, to express an enzyme or an immunogen, nucleic acids encoding the FBFP, vectors containing the nucleic acids, and host cells harboring the vectors. Furthermore, the invention provides methods for obtaining over-expression and surface display of heterologous polypeptides in Gram-positive bacterial cells in Bacillus halodurans in particular. In addition, the invention features gene-disrupted bacterial are useful for expressing the recombinant FBFP on their surfaces. Also included in the invention genetic constructs are useful for making FBFP and methods of using the FBFP.
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