Invention Grant
US07888086B2 Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule
有权
通过与固定化的脱卤酶底物结合并直接或间接连接到蛋白质或分子的突变体脱卤酶固定蛋白质或分子的方法
- Patent Title: Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule
- Patent Title (中): 通过与固定化的脱卤酶底物结合并直接或间接连接到蛋白质或分子的突变体脱卤酶固定蛋白质或分子的方法
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Application No.: US12220478Application Date: 2008-07-24
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Publication No.: US07888086B2Publication Date: 2011-02-15
- Inventor: Aldis Darzins , Lance Encell , Tonny Johnson , Dieter Klaubert , Georgyi V. Los , Mark McDougall , Keith V. Wood , Monika G. Wood , Chad Zimprich
- Applicant: Aldis Darzins , Lance Encell , Tonny Johnson , Dieter Klaubert , Georgyi V. Los , Mark McDougall , Keith V. Wood , Monika G. Wood , Chad Zimprich
- Applicant Address: US WI Madison
- Assignee: Promega Corporation
- Current Assignee: Promega Corporation
- Current Assignee Address: US WI Madison
- Agency: Casimir Jones, S.C.
- Main IPC: C12N11/00
- IPC: C12N11/00 ; C12N9/14 ; C12Q1/34 ; C12P21/02 ; C08H1/00 ; C07C17/00
Abstract:
A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
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