A recording medium, a method of creating a file of the recording medium, and a method and apparatus for reproducing the same are disclosed. In the recording medium including a plurality of recording layers, the method of creating a file of the recording medium includes continuously recording a stream data file within a first recording layer and a second recording layer among the plurality of recording layers, which are adjacent to one another, and forming a PlayList file so as to enable seamless playback of the recorded stream data file, wherein the PlayList file includes a presentation start time (IN-Time), at which playback begins, positioned in the first recording layer, and a presentation end time (OUT-Time), at which playback is ended, positioned in the second recording layer.