Methods of making analyte sensors for determining the concentration of an analyte, such as glucose, in a biological fluid such as blood or serum, using techniques such as coulometry, amperometry, and potentiometry are provided. The sensor includes a working electrode and a counter electrode, and may include an insertion monitoring trace to determine correct positioning of the sensor in a connector. The methods provide a sensor that is calibration-adjusted, eliminating the need for a user to enter a calibration code or for the meter to read a calibration code.