Invention Grant
US07897381B2 Uncoupling of DNA insert propagation and expression of protein for phage display 有权
DNA插入片段的解偶联和蛋白质的表达用于噬菌体展示

Uncoupling of DNA insert propagation and expression of protein for phage display
Abstract:
The present invention provides an advance in phage display technology by permitting the uncoupling of the propagation of phages containing inserted sequences encoding heterologous polypeptides from the expression of said polypeptides. The invention provides phage constructs and methods for their use to permit phage coat protein expression, and thus phage propagation, in the absence of display of heterologous polypeptides, which may be expressed as a fusion with said coat protein in a regulated manner.
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