Invention Grant
US07897381B2 Uncoupling of DNA insert propagation and expression of protein for phage display
有权
DNA插入片段的解偶联和蛋白质的表达用于噬菌体展示
- Patent Title: Uncoupling of DNA insert propagation and expression of protein for phage display
- Patent Title (中): DNA插入片段的解偶联和蛋白质的表达用于噬菌体展示
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Application No.: US11982392Application Date: 2007-10-31
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Publication No.: US07897381B2Publication Date: 2011-03-01
- Inventor: Pietro Ciceri , Patrick Parvis Zarrinkar , Daniel Kelly Treiber , David J. Lockhart
- Applicant: Pietro Ciceri , Patrick Parvis Zarrinkar , Daniel Kelly Treiber , David J. Lockhart
- Applicant Address: US CA San Diego
- Assignee: Ambit Biosciences Corporation
- Current Assignee: Ambit Biosciences Corporation
- Current Assignee Address: US CA San Diego
- Agency: Jones Day
- Main IPC: C12N15/11
- IPC: C12N15/11 ; C12N15/70 ; C12N15/33 ; C12N15/62 ; C12N7/01 ; C12Q1/68 ; C07H21/04

Abstract:
The present invention provides an advance in phage display technology by permitting the uncoupling of the propagation of phages containing inserted sequences encoding heterologous polypeptides from the expression of said polypeptides. The invention provides phage constructs and methods for their use to permit phage coat protein expression, and thus phage propagation, in the absence of display of heterologous polypeptides, which may be expressed as a fusion with said coat protein in a regulated manner.
Public/Granted literature
- US20080318298A1 Uncoupling of DNA insert propagation and expression of protein for phage display Public/Granted day:2008-12-25
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