Invention Grant
- Patent Title: Growth of neural precursor cells using umbilical cord blood serum and a process for the preparation for therapeutic purposes
- Patent Title (中): 使用脐带血清的神经前体细胞的生长和用于治疗目的的制备方法
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Application No.: US11981393Application Date: 2007-10-31
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Publication No.: US07897388B2Publication Date: 2011-03-01
- Inventor: Prathibha Shetty , Chandra Viswanathan
- Applicant: Prathibha Shetty , Chandra Viswanathan
- Applicant Address: IN Rabale, Navi Mumbai, Maharashtra
- Assignee: Reliance Life Sciences Pvt. Ltd.
- Current Assignee: Reliance Life Sciences Pvt. Ltd.
- Current Assignee Address: IN Rabale, Navi Mumbai, Maharashtra
- Agency: Lackenbach Siegel, LLP
- Agent J. Harold Nissen
- Priority: IN196212 20030416
- Main IPC: C12N5/00
- IPC: C12N5/00 ; C12N5/071 ; C12N5/074 ; C12N5/078 ; C12N5/0789

Abstract:
This invention is concerned with stem cells derived from umbilical cord blood serum and a method for growing human embryonic stem cells and adult cells comprising sera separated from clotted umbilical cord blood, including growing and differentiating cord blood stem cells into neural precursors, comprising transdifferentiating CD34+, CD45+ and CD133+ stem cells from mononuclear cells derived from umbilical cord blood to neural precursors. The stem cells obtained from the umbilical cord include pluripotent stem and progenitor cell population of mononuclear cells, and separating pluripotent stem and progenitor cell population of mononuclear cells obtained from the umbilical cord blood. A magnetic cell separator is used to separate out cells which contain a CD marker and then expanding the cells in a medium containing retinoic acid as a differentiating agent supplemented with one or more growth factors BDNF, GDNF, NGF and FGF in presence of cord blood serum. The invention is also concerned with the transplantation and repair of nerve damage, strokes, spinal injury, Parkinson's and Alzheimer's, prepared with a media for culturing umbilical cord blood stem cells in umbilical cord serum.
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