Invention Grant
US07897737B2 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
有权
3'-OH未封端,核苷酸和核苷,用可光裂解修饰的碱基,终止基团及其用于DNA测序的方法
- Patent Title: 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
- Patent Title (中): 3'-OH未封端,核苷酸和核苷,用可光裂解修饰的碱基,终止基团及其用于DNA测序的方法
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Application No.: US11567189Application Date: 2006-12-05
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Publication No.: US07897737B2Publication Date: 2011-03-01
- Inventor: Weidong Wu , Vladislav A. Litosh , Brian P. Stupi , Michael L. Metzker
- Applicant: Weidong Wu , Vladislav A. Litosh , Brian P. Stupi , Michael L. Metzker
- Applicant Address: US TX Houston
- Assignee: LaserGen, Inc.
- Current Assignee: LaserGen, Inc.
- Current Assignee Address: US TX Houston
- Agency: Fulbright & Jaworski LLP
- Main IPC: C07H19/00
- IPC: C07H19/00 ; C07H21/00 ; C07H19/04 ; C07H19/20

Abstract:
Provided are novel nucleotides, nucleoside, and their derivatives described herein, that can be used in DNA sequencing technology and other types of DNA analysis. In one embodiment, the nucleotide or nucleoside with an unprotected 3′-OH group is derivatized at the nucleobase to include a fluorescent dye attached via a linker to a photocleavable terminating group. The photocleavable-fluorescent group is designed to terminate DNA synthesis as well as be cleaved so that DNA oligomers can be sequenced efficiently in a parallel format. The design of such rapidly cleavable fluorescent groups on nucleotides and nucleosides can enhance the speed and accuracy of sequencing of large oligomers of DNA in parallel, to allow rapid whole genome sequencing, and the identification of polymorphisms and other valuable genetic information, as well as allowing further manipulation and analysis of nucleic acid molecules in their native state following cleavage of the fluorescent group.
Public/Granted literature
- US20080132692A1 Photocleavable labeled nucleotides and nucleosides and methods for their use in DNA sequencing Public/Granted day:2008-06-05
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