Invention Grant
US07897835B2 Expression vectors pSSA-K and pSSA-H for expression of SOD and APX in plants to provide stress tolerance
有权
表达载体pSSA-K和pSSA-H用于在植物中表达SOD和APX以提供胁迫耐受性
- Patent Title: Expression vectors pSSA-K and pSSA-H for expression of SOD and APX in plants to provide stress tolerance
- Patent Title (中): 表达载体pSSA-K和pSSA-H用于在植物中表达SOD和APX以提供胁迫耐受性
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Application No.: US11718661Application Date: 2005-01-28
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Publication No.: US07897835B2Publication Date: 2011-03-01
- Inventor: Sang-Soo Kwak , Suk-Yoon Kwon , Haeng-Soon Lee , Li Tang , Soon Lim , Byung-Hyun Lee
- Applicant: Sang-Soo Kwak , Suk-Yoon Kwon , Haeng-Soon Lee , Li Tang , Soon Lim , Byung-Hyun Lee
- Applicant Address: KR Taejeon-Si
- Assignee: Korea Research Institute of Bioscience and Biotechnology
- Current Assignee: Korea Research Institute of Bioscience and Biotechnology
- Current Assignee Address: KR Taejeon-Si
- Agency: Lucas & Mercanti, LLP
- Priority: KR10-2004-0093945 20041117
- International Application: PCT/KR2005/000258 WO 20050128
- International Announcement: WO2006/054815 WO 20060526
- Main IPC: C12N15/82
- IPC: C12N15/82 ; A01H5/00 ; C07H21/04
Abstract:
The present invention relates to a recombinant expression vector, pSSA-K or pSSA-H, containing oxidative stress inducible promoter, such as sweetpotato peroxidase anionic 2 (SWEPA2), a nucleotide encoding superoxide dismutase (SOD) and a nucleotide sequence encoding ascorbate peroxidase (APX) linked to the promoter, antibiotics resistant gene, tobacco etch virus leader sequence, a polynucleotide encloding a transit peptide for chloroplast targeted expression, CaMV 35S transcription terminator, and T-DNA boarder sequence, and a method for preparing multiple stress tolerant transgenic plants using the recombinant expression vector.
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