Provided is an apparatus and method for controlling multi-channel access in USN-based MAC. The multi-channel access control apparatus includes a channel scanner, a control channel preoccupier, and an available channel exchanger. The channel scanner scans at least one or more available channels among the N data channels in a Tx sensor node. The control channel preoccupier preoccupies, in the Tx sensor node, the control channel through control channel sensing and back-off and broadcasts a preamble on the control channel periodically. The available channel exchanger detects, in an Rx sensor node, the available channels available to the Tx sensor node on the basis of the received preamble, selects a channel available to the Rx sensor node among the detected available channels, and transmits information about the selected channel to the Tx sensor node.