A method is disclosed for segmentation of anatomical structures, in particular of the coronary vascular system, from a sequence of 3D image data records recorded in a time sequence, in which the anatomical structure is first of all segmented from a first of the 3D image data records. In the method, during the segmentation of the anatomical structure, search areas of the segmentation are restricted and/or segmentation parameters associated with the three-dimensional relationships from the other 3D image data records are used, on the basis of known spatial conditions of the structure to be segmented, with respect to anatomical objects which are located in the surrounding area and of results of the segmentation from a respective next 3D image data record in the sequence from which the structure has already been segmented.