According to one embodiment a method of making optical fibers comprises: (i) manufacturing a core cane; (ii) situating a plurality of microstructures selected from rods, air filled tubes and glass filed tubes and placing said microstructures adjacent to the core cane, said microstructures forming no more than 3 layers; (iii) placing the core cane with said adjacent microstructures inside a holding clad tube; and (iv) placing interstitial cladding rods inside the holding (clad) tube, thereby forming an assembly comprising a tube containing a core cane, a plurality of microstructures and interstitial cladding rods. The assembly is then drawn into a microstructured cane and an optical fiber is drawn from the microstructured cane. According to several embodiments, the method of making an optical fiber includes providing at least one air hole and at least one stress rod adjacent to the core.