An invention is disclosed for locating a region of interest in the calcaneus. A pair of ultrasound transducers are positioned on the medial and lateral sides of the heel, respectively. The positioning is based on a size of a portion of the body of a subject upon whom the ultrasound assessment of the calcaneus is to be made. In a presently preferred embodiment, the length of the foot from the back to the head of the first metatarsal is used in conjunction with a proportionality constant and an angle, to position the pair of transducers. The positioning so obtained facilitates (i) reproducible measurements and (ii) comparisons of the results obtained in one person with another, because relatively analogous portions of the highly heterogeneous calcanei are assessed in both. In an alternative embodiment, a single transducer is positioned similarly on the heel, operating in pulse-echo mode.