Invention Grant
- Patent Title: Variant lipid acyltransferases and methods of making
- Patent Title (中): 变体脂质酰基转移酶及其制备方法
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Application No.: US11852274Application Date: 2007-09-07
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Publication No.: US07906307B2Publication Date: 2011-03-15
- Inventor: Jørn Borch Søe , Jørn Dalgaard Mikkelson , Arno de Kreij
- Applicant: Jørn Borch Søe , Jørn Dalgaard Mikkelson , Arno de Kreij
- Applicant Address: DK Copenhagen
- Assignee: Danisco A/S
- Current Assignee: Danisco A/S
- Current Assignee Address: DK Copenhagen
- Agency: Frommer Lawrence & Haug LLP
- Agent Thomas J. Kowalski; Heather J. DiPietrantonio
- Priority: GB0330016.7 20031224; GB0415999.2 20040716; GB0716126.8 20070817
- Main IPC: G01N33/53
- IPC: G01N33/53 ; C12Q1/34 ; C12N9/00 ; C12N9/16 ; C12N9/10 ; C07K14/00

Abstract:
The present invention relates to a method for the production of a variant lipid acyltransferase comprising the steps of: (i) selecting a parent enzyme which is a lipid acyltranserase enzyme; (ii) modifying one or more amino acids to produce a variant lipid acyltransferase; (iii) testing the activity of the variant lipid acyltransferase on a galactolipid and/or phospholipid and/or triglyceride substrate; (iv) selecting a variant enzyme with enhanced activity towards galactolipids compared with the parent enzyme; (v) providing a Bacillus licheniformis cell; (vi) transforming the Bacillus licheniformis cell with a heterologous nucleotide sequence encoding said variant lipid acyltransferase; and (iii) expressing said variant lipid acyltransferase in the cell under the control of a promoter sequence. The variant lipid acyltransferase can undergo post-translations modification, truncation and/or clipping, i.e., to remove a signal peptide. In addition, the present invention further relates to the use of Bacillus licheniformis to express a lipid acyltransferase, a Bacillus licheniformis host cell comprising a heterologous lipid acyltransferase and a vector comprising a nucleotide sequence encoding a lipid acyltransferase operably linked to a promoter sequence homologous to B. licheniformis.
Public/Granted literature
- US20080070287A1 VARIANT POLYPEPTIDES AND METHODS OF MAKING Public/Granted day:2008-03-20
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